The Peptide Secrets to a Youthful Face : 1536

0:00Part of living to at least 180 or as long as you choose is looking like yourself. There is studies showing that the skin just under or above your eyes are 25 to 30 years older than the skin on your cheek. As they go through menopause, their skin seems to be failing apart. They can hold water anymore. Now, if gelp1, we have seen so many people using our body lotion and really helping reducing the sagging and firming the skin through menopause. Women start to experience crepness and the skin gets thinner. My massage therapist is like, why your skin is changing? So we saw 93% of the participants in improvement in firmness and 97 improvements in fine lines and wrinkles. And we compared to if a rubber mycin and rubber mycin was not able to reduce the biological age in a significant way. When we treated skin with we saw a reduction in 2.5 years in the biological age of the skin.

1:00No one was doing this, you know, back almost 10 years ago when we started this research. And we generate another 800 peptides and we pass it again and then eventually fall. There is a low level of inflammation coming from your skin that's actually impacting your body. But if you can start using this as early as possible, you can prevent the loss of structure in declining collagen. You're listening to the human upgrade with Dave Asprey. Have you hit your protein goal today? Well, you might, but it's probably not good protein. And butcher box has made it easier to get good protein for your protein goal. They deliver 100% grass fed beef and organic chicken and wild-cot seafood and a bunch of other good stuff right to your door. They've already done the research and they made the tough sourcing decisions. So you don't have to go to the store and ask questions that the butcher can't answer anyway. That means all you have to do is decide what's for dinner. Every butcher box is customizable so you can choose the cuts

2:00and the proteins that fit your routine and just what you like to eat. Busy weeknight or friends coming over, you've already got great protein ready to cook. My days are really busy so it's easy to just throw one of the grass fed steaks on the grill after I work out. And if you're looking for an easier way to make great meals, give butcher box a try. Go to butcherbox.com slash human upgrade to get $20 off your first box plus your choice of free ribeye, New York strip, or filet mignon in every box for a year with free shipping always. Again, that's butcherbox.com slash human upgrade. Be sure to use our links so they know we sent you. Why won't your stress budge no matter what you do? You're doing the work, the cold plunge, the breath work, the supplements, and your nervous system is still running hot. Here's the problem. Every recovery tool you use only works when your body believes the threat is over. And if your nervous system never gets that signal, it stays in survival mode regardless of what you stack on top of it. The direct line to that signal runs through your Vegas nerve. When you stimulate it, your parasympathetic nervous system

3:03activates. Your body shifts out of fight or flight and into the state where real recovery happens. Zenbud is the tool I reach for to make that shift happen. It sits on your ears like a headset and uses focused ultrasound to reach your Vegas nerve directly. Just five minutes daily is all you need. In clinical studies, participants experienced significant improvements across stress, mood, and sleep after just four weeks of use. Many people notice it in their first session. Check it out at zenbud.health and use code DAVE15 at checkout for a discount. Your biohacks are only as good as your nervous system's ability to recover. This is the missing piece. Part of living to at least 180 or as long as you choose is looking like yourself. And that means taking care of your skin. Taking care of your skin is different for men and women. And it's different depending on how old you are chronologically and biologically. Some people have much younger skin, some people have older skin. So how about we have a deep conversation with a PhD in stem cell biology and tissue engineering. Sounds like a good

4:10way to learn about skin, doesn't it? That's because our guest is the CEO and co-founder of One Skin, a company that actually created a new peptide. Wait, can we say peptide without getting beat? We should probably bleep that one out depending on if we're on certain platforms. Anyway, a new series of amino acids that are stuck together to be a signaling molecule that shall not be called a bleep because they're censoring people who talk about bleep. Now, I could be making a big deal of that, but I've been banned twice for very tame reporting of science on that. So I'm a little sensitive here. But it's funny on X, they never seem to, never seem to ban me for anything. It's kind of weird. So, Co-Inventor 5 patents published in multiple journals and a person who's made a lot of the products that I use on my hair and on my skin, none other than Carolina raised De Oliveira. Welcome back, Carolina. Hi, Dave. Thanks for having me again. Last time we talked about hair and scalp and you

5:18kind of blew me away with the product I was already using it. I used the one skin product for my hair regularly because it's so thick and the woman who cuts my hair does a lot of celebrity, like A-listers, happens to live in town. Thanks, Nico. She's like, what are you doing for your hair? This is insane. It just keeps growing. She keeps having to come out and cut it. Now, she says, I use one skin on all of my clients. She has a lot of women like, my hair is thinning and so I think she might be your biggest fan. That's awesome. I was so happy to hear. We have seen great success and a lot of people sharing that. Me included, I have so many new hairs. It is something that requires the consistency. So you've probably been using it for months, right? For those who keep the consistency, you will definitely see in your growth. That's super exciting. It's a huge problem for those who

6:20experiencing hair thinning, it really impacts your self-esteem in a very deep way. We're happy to help people with this problem. What's new? We talked about skin on the first one and the effect on wrinkles of this. We talked about skin. I'm talking about hair. What's new? Yeah, I think the area that we've been interested in in the past two years is the loss of hydration that happens with aging and mainly we've been very curious about this topic because a few things. When your skin is less hydrated, your wrinkles will look deeper. Your texture will look less smooth and you're going to feel you're going to look a little older and less rest in general. There is the aesthetics piece of it, but there is also a sign that your skin is not functioning as well because as we know, the main function of the skin is to be a barrier. If it's losing water,

7:24the barrier is probably being compromised. On top of that, we have been hearing so much from women in our community that as they go through menopause, their skin seems to be failing apart, like they can hold water anymore and then they are going through a lot of these issues and can't understand why this is happening. We got very interested in learning how we could support women that are going through this problem. Obviously, men can go through this as well. There is a bigger impacting to women because of the fast decline that happens in hormones with menopause. We spend two years, the past two and a half years just researching how we can support this biological problem that's affecting women as they go through this aging process. With women, you hit paramanopause and all of a sudden your skin is dry again a couple of years. What just changed? With guys,

8:26it's like a 20-year decline because when we have more testosterone to make more sebum and our skin is more oily. You're not going to notice a 20-year decline and at some point you might get dry skin. I certainly don't get dry skin but I also use one skin and a bunch of other stuff on my face and so I have well hydrated skin. Is hydration oil or is it water? It's both. Actually, the skin has a beautiful way to maintain, to hold water and to prevent water loss. Maybe we can just explain how this works. Just like refreshing the main structure of the skin. From inside out, you have the dermal layer that's the layer that provides the firmness that has the fibroblasts, the chrysanctum collagen and then provides this main architecture of the skin. And then you have the epidermal layer that's mostly made of keratinocytes. These keratinocytes will differentiate and you start accumulating keratin and eventually the upper

9:31cells they expel the nucleus, they accumulate a lot of keratin and they become cornucytes. And these cornucytes are the cells that are going to hold water. They produce what we call like natural moisturizing factors and they can hold water. But on the outside, you have this layer of lipids that's also secreted by the upper layers of the keratinocytes of the epiderms. And this is mainly composed by ceramides, fatty acids and cholesterol. So now on the outside, you have this water barrier layer that will prevent the water loss. So we usually call this system like a brick and mortar because the cornucytes are the bricks that are holding water inside through these natural moisturizing factors and the mortar is basically this layer of lipids that prevent water from escaping. So it's a very beautiful orchestrated process and what happens with

10:33aging, a lot of things happens with aging, but your skin lose this ability to create this is stratum cornuc, the brick and mortar in a way that's functioning well and you start to have like this process compromised. And that means that you lose water because the layer of ceramides and fatty acids doesn't work anymore. And because the ore yogurt also cannot hold water as well, you're not producing. So there are a lot of things that can disrupt that. If your cells you're not producing the cornucytes or not producing enough of this NMS, the natural moisturizing effects you can hold water. And if there are breaks in this lipid layer that you know, you lose more water. Okay, what do you do about it? Yeah, so what happens a lot of the products out there when they are trying to provide moisture, most products are focusing only on delivering like water or humectants or products or ingredients that can hold water or also ingredients that are

11:37occlusive. So creating a barrier on top of your skin. So you're trying to prevent water loss by creating a barrier or by delivering ingredients that are going to attract water. What we should do about it is two things. One, stimulate your skin to produce that barrier again. So produce the natural moisturizing factors, build that ceramide and lipid barrier again. And on top of that, there is this internal architecture that holds water inside the skin that's made of hyaluronic acid. And in order to have this structure to function well, you also need more collagen and you also need elastene. So on the dermal layer, you also have this decline on this structure that prevents your skin from holding water. So you need the two things. One, it's a system that allows the skin from

12:38holding water internally. And another one is preventing water loss at the barrier level. Okay, so the synthetic way that a lot of companies are trying to keep things in is like putting a piece of saran wrap over the skin. Yeah, kind of. Let's seal it in with silicone or something. Yeah. So that it's sealed. Now that will keep watering, but then the skin doesn't function like it's supposed to get weaker over top. Yeah. As I understand it anyway. Okay, so that's one thing. Your approach is let's do what it takes to make the skin create its own barrier that's actually more flexible and more permeable than a synthetic layer would be. Yeah, we're going to both help the skin to create its own barrier and help build that architecture internally that will hold water at the dermal layer of the skin as well. Now the ingredient that I like for holding holding water in tissues is well, creating obviously, but that's less of a skin thing because creating helps your cells have more water, which makes ATP better. And there's a little known

13:42sugar called tree halos, which absorbs water very beautifully. So you can take that internally. Some people put it in, topically, in ingredients, but the problem there is that's also like an artificial way to make that water come in. So with one skin, how do you make the cells hold more water? Like what's the trick? Well, so there are two ways that we can do that. We know that part of what's happening that's causing this loss of hydration is one is the accumulation of this senescent cells. So as you have more senescent cells in your skin, you're going to have less cells producing collagen, producing the structure that holds the water together. You're going to have more inflammation that it's basically needed to protein to enzymes that will degrade collagen. So you have collagen, you have hyaluronic acid, and so you're losing this system that's working

14:43well inside the skin. And then obviously as we're talking with menopause, there is this huge decline of estrogen, and then this process gets accelerated, gets aggravated. So you have even more inflammation, the estrogen that was being supposed to prevent the collagen degradation is simulated that lipid barrier production is all being compromised. So the way that we're doing, we're solving this problem is primarily with our peptide that is going to help like reducing the amount of senescent cells, reduce the inflammation. And we created the peptide in a formula that's now supporting collagen production, is supporting a less in production. Hyaluronic acid production, we know that hyaluronic acid is the key molecule to hold water internally. Right. And we also showed that as you restore this internal system, this internal architecture,

15:44you are now leading to good signals for the epidermal layer to do its job, to continue creating the barrier that needs to create. So we have a lot of interesting data showing how we could address both like the dermal layer in terms of the structure, plus the epidermal layer in terms of forming the barrier in our studies in the lab. Now this is the same peptide that I've been using from the last five years for months again. So what did you change in the formulas so that now it's doing collagen and hyaluronic acid? So it also does that in our other product, the difference here is that we combine with other ingredients that will provide this immediate hydration, so a deeper hydration. So we have ingredients that will support that are humectants, that will help create like a lipid barrier on top of your skin. So we are delivering this kind of immediate relief in terms of preventing the water loss while the peptide is doing its work. And every

16:49formula is different because the way that we combine the peptide with other ingredients, it completely changed how the formula works. So in this case we built a formula that can support all of these different parts of the mechanisms that are important for hydration. So we want to support this skin to create its own natural moisturizing factors. We want to support this skin. We are delivering like ceramides, cholesterol and fatty acids. We are also helping, we have ingredients that are forming this lipid barrier, so we will prevent the water loss. And then on top of that we have other ingredients that are antioxidants that are soothing. So all of that with the peptide, we can now deliver both that need of like preventing the water loss and deeply hydrating the skin. But also allowing your skin to rebuild this process is that are important

17:51to hold water and again build the barrier that prevents the water loss. I've seen a few brands put collagen into a topical formula. Does that work? So we actually use collagen amino acids because the amino acids are very high-grossed topic, so they hold water really well. It's actually one of the natural moisturizing factors that we have in our cells. So we do have collagen amino acids alongside. Like glycine and proline. Yeah, it's a combination of those amino acids, yeah, that helps hold water. That makes sense because you can use amino acids, but collagen is too big of a molecule. Yeah, exactly. It's not that you're adding collagen on top of that your skin suddenly has more collagen. That's why in order to make your skin to produce collagen, we almost needed to go to this source. Why your skin is stopping producing collagen, right? Right. And it always kind of makes me laugh. There's a cap on the end of each collagen molecule. And with that cap in place, it will not enter

18:56the skin. And if you take the cap off, it degrades quickly. So it has to be frozen or refrigerated. And maybe there's some high-end place in Switzerland or something that has that fridge and charges a lot for it. But you're not going to add collagen to the surface of the skin. You can eat collagen, which I kind of made at a billion-dollar industry 10 plus years ago, because there's a case for it. And then it breaks down to amino acids. And it even makes a signaling molecule in your eat collagen that tells the body of the skin has been injured when it hasn't. So it'll make thicker. Yeah. But if you're using one skin that has the amino acids on the surface that are holding the water in, the ones that are the precursors, that seems like a really good product. Yeah, yeah. You're giving yourself the precursors so the cells now have the substrate that they need to build its own collagen. Or to hold more water if it's the amino acids. And you actually put cholesterol in it? Yeah, there is cholesterol in the formula. Oh no, I thought cholesterol would fit. This is why when women eat egg yolks, your skin starts to glow because your body needs cholesterol.

19:59Cholesterol is so important. And as your skin is aging, it declines in cholesterol. And it's one of the key pieces that your cells need to build at that strong barrier. So if I wanted to look really old, I should lower my cholesterol levels as much as possible, right? Well, I don't know about that. I mean, I do it. It's science here. Right? Cholesterol is a necessary molecule in the body. Just make sure it's not oxidized. Yeah, exactly. Yeah. Yeah. And maybe have less of those zombie cells or senescent cells putting out. How do you get rid of senescent cells in your skin? So in our case, our peptide, it works not as senolytic molecule in a way that to kill the senescent cells itself. It works as what we call xenomorphic, basically in two ways. So for the cells that are ready senescent, that are ready to produce inflammation that are basically deteriorating the tissue, we shut that cell off. So it's not like spreading the inflammation. And then for the cells that are

21:03in the early stage senescent cells, we activate their DNA repair system. So it doesn't become late senescent cells when it's like really spread inflammation. So what I happen is that when your tissue, your skin has lower inflammation, your own immune system can clear up those senescent cells that are already there because the immune system is not overwhelmed by the inflammation that was there before. So it's a very natural way to allow your skin to function as it should, because now we are reducing the inflammation. We are activating the ability of the cells to repair itself. And then the immune system can clear out the senescent cells that are there. And it's not that it clear all the senescent cells out there. We still need some and we know that senescent cells are important for process like wound healing, but what we don't want is an overload of senescent cells, because then there is more inflammation. And we know that inflammation basically leads to this

22:05tissue deterioration. Wow. So you can actually find an early stage zombie and stop it. Yeah. You're ruining all the zombie movies right now. You can go, you can convert them back. Yeah, zombie bites you. I'll just take the antidote. That's not fair. You can have an entertainment. But for longevity. It's good. Yeah. That's really good. Yeah. Have you ever experimented with using your peptide LS1 or early or internally injected any of that kind of stuff? Yeah. No, not yet. So we, I think it's such an interesting and powerful molecule, but we would need to do a lot of safety studies in animals. And because our primary focus is in the skin right now, we don't want to go there. Can you just like test on employees? I can give. I was going to joke. I can't give them to you. No, we're not going to tell you no place. But it is an interesting molecule. And

23:06interesting enough, like we have compared with GHK copper. And we've seen obviously GHK copper is so popular right now. And we see better collagen production from our peptide than GHK. Another thing about GHK copper is that depending on the supplier, it doesn't work. Like we test five different suppliers. Some, yes. So there is the oxidation that can happen. So there are a lot of things that for peptide to actually be bioactive and do its job in it should be stable, in it should be able to penetrate the skin. And so is that GHK is very polar? No, so how you're going to make it go through this lipid barrier? So I think it needs to be stable at different temperatures, pH. So checking out those buckets for peptide to go into the deeper layers of the skin and do its work is not simple. I know that everyone is excited about adding a lot of peptides in creams and saying this has the most amount of peptide ever. And based on what we test in the lab, having

24:12more is not necessarily better. Sometimes they will interact with each other in a way that you can't predict. And we know because we're testing the skin in the lab, so we see how the skin responds. So it's a very fine tuning process and it's not like, you know, put more, it's better. Okay. So the right dose might be important for peptides? The right dose and the right combination. Like what else that peptide is around? What else? What are the ingredients in that formulation? Okay. Your longevity stack depends on one system you probably don't think about enough. It's the one responsible for getting the goods to where they're going. Every nutrient, every hormone, every supplement has to travel through your blood vessels to do its job and two things determine whether your vascular system actually works. The first thing is your endothelial glyco-calix. Say that three times fast. It's a slippery layer on the inside of your blood vessels and it's the first land of events for every vein and artery in your body. And the second thing is nitric oxide. That's the molecule that lets blood vessels relax and open so nutrient synoxygen can actually

25:16reach their targets. That's the reason I take arterousyl HB and vasconox HB from Caleroihel Sciences every single day. Arterousyl HB supports that inner blood vessel lining. And vasconox HB supports nitric oxide production for up to 24 hours. Open label published research backs these formulas. Arterousyl HB and vasconox HB are the perfect combo for vascular health. You're already doing the work. Now make sure the system carrying all of it is ready to deliver. This is what I actually do every single day. Go to caleroihel.com slash day to get a discount. Now's a good time to commit to the things that make you feel better, feel more powerful. And part of that is upgrading your hair and skin routine. If you're thinking about having better hair, maybe this is your sign to start. The iris store elite and aluminum face mask are both part of my stack. The elite is their advanced hair growth system. It's got 300 lasers and 200 LEDs to help your growth thicker, healthier hair in about three months. And it's really convenient and you can wear it when you're working or reading or cooking or doing anything else. And the aluminum face mask

26:18takes about 10 minutes a few times a week. It gives you red, infrared, and even a little bit of blue light which you want on your skin in limited doses. The result is fewer breakouts, smoother skin, and less dullness. And I restore back to products with a 12 month money back guarantee. So if you don't see results, you get a full refund, even if you used it. I restored offering some huge discounts on the red light therapy devices right now. You can save on customer favorites like the iris store elite helmet and the aluminum face mask. Go to irisstore.com slash day. And I appreciate you supporting the show and telling them that I send you there. So give your hair and skin the upgrade that deserve so you look the way you want. That's iris store dot com slash Dave. I do think if you can get GHK in the right quality into the skin, there's pretty good evidence for it. Yeah. But your point is that most of the time people put it on and it doesn't go into the skin. Yeah. Okay. And then you did compare your peptide, the OS one with GHK to look at which one had better longevity even when it goes into the cell. Yeah. What did you find when you compare

27:21yeah. These are like preliminary studies. But like the main thing that we were looking safe GHK would increase collagen. And as I said, in some case, it did not have peptide produced in those more collagen than the GHK. We want to go back and and study it again. But from our initial screening and we test like five different suppliers initially, I think four out of five didn't work. So yeah. So there is a lot that we need to be careful about again, the quality, the stability, if that molecule can oxidize. And you tested hundreds of different peptides and molecules. Talk about how you did that originally to come up with this novel peptide that you have. Yeah. So initially, we had a library of antimicrobial peptides. And we had these hypotheses of let's see if any of these peptides can reduce the MSN cells in the skin. We think that if we target MSN cells, we could make our skin behave

28:22like younger skin. No one was doing this, you know, back almost 10 years ago when we started this research. So we did the first round of screening in cells that we use two types of cells. One is cells derived from progeria donors. These are, you know, it's a genetic mutation that the kids age really fast. So they accumulate a lot of MSN cells. Oh, we got cells from elderly donors like 70, 80's years old. And we put the peptides in contact with the cells and we measure how many MSN cells were there. We did this first round. We got four peptides that worked well. We used an algorithm that now we are creating optimization on these sequences. And we generate another 800 peptides and we test it again. And then eventually found OS1. And OS1 was able to reduce almost 40% the load of MSN cells. And when we measured the biological age, when we treated

29:24skins with OS1, we saw a reduction in 2.5 years in the biological age of the skin. And we compare to a verapamysine, which you know, it's a very known like senolytic. And verapamysine was not able to reduce the biological age in a significant way. So it was very interesting to say that by reducing senescent cells, we reduce inflammation, we reduce markers like IEL6, IL8. We reduce metallic proteinases. These are proteins that degrade collagen. And we allow the cells to produce more collagen. And in turn, what you have is a skin that's now functioning like biologically younger skin. Wow. That's incredible. Okay. I'm kind of lazy. I'm a guy. So how many different products are you going to put on my face here? So our goal is always like to simplify people's skin care routine. We don't think that anyone is like 10 steps skin care routine. That's why

30:32even though we're launching new products, you can still pick and choose what you think that's best for you. So for example, now that we have this rich cream, that will be really important for women that have more mature and dry skin, they can probably use OS1 face in the morning and use OS1 reaching the evening. So for you, I would say you can continue using OS1 face when the climate is drier or if you're traveling for a skin and it's really, really dry out there, really cold, you can use the rich moisturizer. So I should bring the rich moisturizer to Burning Man. Yeah, desert. Okay. Yeah. I'll definitely. I'll smear it on and then the dust will immediately stick. Oh yeah. No, this when you're sleeping, if you can wash your face and then you sleep. Yeah, that's probably how I would do it. And it's funny. I actually do bring OS1 the one skin

31:33when I go to Burning Man because it is like a brutal environment for everything. Yeah. Yeah, your skin doesn't crack at all. It's so beautiful. The face moisturize because it's a light weight moisturize. It's still going to help you hold water. It's not going to be as deep hydrating as the new product but it absorbs so fast so your skin will look immediately like dry in a way that it's nice. You don't feel the product sitting on top of your skin. And I do want to thank you for not putting weird fragrances in there. Yeah, no fragrances, no artificial fragrances. Yeah, they mess with my eyes and I hate putting stinky stuff on my face. Even if it smells nice. I like know it doesn't. It smells like chemicals or even essential oils. You don't want those in your eyes either. So I've never had any eye problems from one skin and a lot of stuff that I've tried like my eyes get irritated because there's chemicals in there just to make it smell a certain way. I don't want to smell like oranges on my face. Yeah, yeah. We know that fragrances can be

32:36cause allergy. So we always avoid fragrances. Our products are also validated for people that have sensitive skin. So we are certificated by the National Exam Association. So people with XM can use our products. So it's pretty safe. They made when we're testing our products, we also see is the skin looks like looking like stronger, more beautiful is the skin is looking like it's irritated or it's inflamed. And obviously we're always like delivering benefits without causing that, you know, side effect. Is it true that you can over moisturize your skin so it just stops protecting itself? So there are always that you can disrupt that layer. So this cornea sites, these cells that hold water, they can only absorb so much water. So if I've seen people in airplanes sometimes they sleep with like this face sheet and they sleep the whole night. And at some point those cells will like swell

33:36and they will break down. So you're disrupting this by adding too much water. This cells can start to break down so you can compromise that barrier. So I think there is always a balance. And so if you want to use like a face sheet, you use like for 15, 20 minutes, maybe one or two times a week, don't sleep the whole night. Even if our Nair plane that you know, it can be really dry. So there is a balance and you don't want to disrupt that balance. So you can like blow up the balloon to the point that it pops. Yeah, yeah, exactly. The other thing that people may do is they only use this occlusive, you know, layer like Vaseline, there's like this huge trend on like take talk of slugging. And basically you are adding this petroleum based, you know, with the texture is definitely nice. If your skin is really really dry, it can hold like, it can prevent water loss immediately. So it can bring some relief. But again, you know, want to do that forever. You always want to help

34:41your skin to build its own barrier again. So if you need this as an like just a immediate relief, you can do it. But it's not something that people should rely on as a way to add water, you know, to their skin. You're not at all water skin will stop doing what it's supposed to do because it has this like yeah, yeah, I just like to use diesel. It's just like just a little bit of petroleum. Yeah, luckily we have a better, you know, more sophisticated from relations like they need to do that. People are so funny like they'll get a belief that something is good. Like the guy who an invented Vaseline, he ate a tablespoon of it every day. Like oh yeah, this is going to line my gut and make it more healthy. I don't think that's how it works. Yeah. I have a serious question about zombie cells, which is an essence, holes in skin. So you have this xenomorphic ability to take the the beginning zombies, the budding zombies and turn them back into non-zombies. But don't we need

35:45that process of getting rid of these cells? Like is there a downside to having less senescent cells? Again, you don't want to completely eliminate all of them. As I said, you need some senescent cells during a wound healing process. What you don't want is to have a lot of them because you're going to have an excess of inflammation. So why you want to get rid of zombie cells? Because when they're already damaged, they're not supposed to be replicating, otherwise they could become a tumor. So it's a natural way to protect your own body from cancer because those cells should stop the virus. Senescent cells are essentially non-dividing cells. But if they are in the early stage and they have a little bit of damage accumulated, your cells can still repair that damage. So they can become functional again if they are in the early stage. So by preventing that, you're maintaining a lot of your cells in that healthy state instead of needing to complete eliminated them and then

36:48build the new cells. So you have to manage the size of the zombie herd. Yeah, exactly. You need enough zombies to eat the brains, but not too many zombies. Yeah. Maybe if we could make zombies only eat politicians. That'd be so good. Oh, wait, there are already zombies. It wouldn't work. All right, let's get a little bit nerdy about how you measure skin. So a traditional way of having a machine downstairs is you take a very bright photo of the skin and you analyze it with AI and you can see like where's the damage underneath. The dermis are in the dermis and where the wrinkles and the aging spots and all that kind of stuff. And I've got a machine like that because, well, I am a biohacker and because then I can track whether things are working or not. Normally, dermatologists would do that. Thank you Chinese manufacturers of weird stuff that you could buy. So there's that, but you don't do that. You're looking at something called mole clock. What is that?

37:48Yeah. So essentially mole clock is an algorithm that we built to measure the skin's biological age, which essentially means how healthy, how functioning your skin is. So let's say, you know, I'm 40 years old. My skin can either be 35 or can be 45 biologically speaking. It's going to be older if I have accumulated a lot of damage, a lot of some exposure, pollution, if I smoke, and it could be younger than my biological, than my chronological age, if I have like really healthy habits, if I nurturing my skin, preventing that damage. So what this mole clock does is basically measuring, measuring at the epigenetic level, some CPG or like chemical markers that accumulate as our skin ages and giving that skin a biological number that correlates with how

38:50functional that skin is. So it's the most accurate way to validate if a product or if an intervention is working because it's objective. It's measuring again, a depigenetic level, which has broader implications in how the skin functions, but it is considered one of the most accurate ways to validate if we can reverse the biological age of the skin or how certain tissues behave, and then we can obviously compare with their chronological age, in the case of, you know, if we're doing this testing humans, or we can compare with like different treatments to see how different treatments change that biological age. So it's kind of like aging clock, like true age, or glycine age, but specifically for skin cells, so that you can tell whether your peptides working starting inside the cell. Exactly. So the reason why we built mole clock, it's because there was no clock that was specifically trained with the skin samples only. These clocks, they were

39:56either like a mix of like different tissues or skin and blood, that's the one from Harvard, and in order to be most accurate, we trained this clock using skin samples only. So now we have the best way to correlate that biological age and measure interventions that we're testing. Okay. Is there some other companies or other biohackers like mecanias? So right now the only inconvenience of this method is that we still need a skin biopsy, so it's not very friendly, consumer friendly. So we mainly use it for research purposes. So when we're running clinical studies, part of our studies like we collect biopsies in people, but then they already agree with that. How big of a biopsy are we talking about? It's like two or three millimeter. Oh, so it's pretty sizable. Yeah. Yeah. And you get like, to get that from the face, or just like from the bladder. So we depend on the experiment we can get from the inner arm, we can get from behind the ear or this close to the ear here. Ideally, the closest to the face,

41:00the better if we want to obviously test a product for the face, but we have tested it in the arm, just as a way initially to be more, you know, yeah. And I should have saved my skin for you. I had a facial circumcision. Yeah, we could have we could have tested products in your own skin in the life that would be really cool. And for listeners, if you haven't fallen for a while, about three, four years ago, because I was meaningfully obese, like a hundred pounds more than I'm now, I had a piece of skin the size of the cover of your passport on each side of my face, it's just extra skin, you could grab and pull. And that's why you probably don't want to get really fat. So I decided, like no amount of longevity is going to get rid of that. So I cut it, the family you would cut your fingernails and removed it. But I'm just thinking I should have sent it off to you guys, because then you could have had enough to do tests for those skin cells. Yeah, that's well, your face looks amazing. Oh, thank you. I just turned it into a wallet,

42:05do you want to see it? Yeah, it was, it just made me think about that. Because I probably saw some extra skin floating around somewhere. Can people put this rich cream like on the back of their hands? Because this is something that men and women get when they age, you get like more wrinkles on the skin. And you get like a thinning of the skin on the back of the hands. Is that a good use case? You can. Again, the rich cream will help rebuilding some of that architecture that helps your skin looks thicker. Obviously, on your hand, you also lose a lot of fat. So there is, that's something that's a little harder to solve. So right now people are like injecting fillers in a way that you can bring the volume back. But if you can start using this as early as possible, you can prevent that fat loss and that, and you can prevent that loss of structure, the declining collagen.

43:05So that makes sense. If you put the one skin products on, like before you go to bed, there's just some under fingers just on the back of your hands. Yeah, I always do that. Everything that I put on my face, I put on the back of my hands. But in our body lotion, you can also, yeah, you can also use on your hands that's good. Okay. Collagen is the most abundant protein in your body. It makes up your skin, your tendons, your joints, your bones, your hair, your connective tissue. Problem is your body makes less of it as you age. And then you get stressed and you eat some processed food and you get even less collagen. Puries pure collagen peptides make it easy to support your body's collagen. Each scoop gives you 10 grams of hydrolyzed collagen from grass-fed cows with no GMOs, no hormones added. It's unflavored, so you can stir it into your coffee, maybe even danger coffee, and never even think about it again. And what sets Peoria Part is transparency. Every batch goes through third party testing by the Clean Label project. They screen for over 200 contaminants, including heavy metals and pesticides and residual solvents and bisphenols. Then, Peoria publishes the results online. You scan the QR code on the label,

44:11and you can see the test results for your exact batch of collagen. Peoria is the only supplement brand with the Clean Label project transparency certificate. So if you're going to spend money on supplements, look for the proof. Go to puroi.com slash day to get 32% off your first and you start a subscription. You'll save more than 26 bucks. That's puri.com slash day. Now, there's some bros out there right now going, what do you care about your skin? Because bro, I don't want to look old when I'm 180. That's why I take care of it. And your skin actually has a function in the body. It's not just for cosmetics. So there's the idea that I'm not going to take care of my skin because I'm tough. Yeah, that's the same mindset that says I'm going to drink a six pack every night because I'm tough. Yeah, you're very tough. I really respect you. And thanks for exhibiting what not to do. So I'm very open about taking good care of my skin. I also don't obsess with 85 different. Yeah, sometimes that's not even good for you.

45:16So I do have a confession to make. Yeah. Okay. So you send me some yourself and I also am on subscription with one skin. So I get the facial stuff. Someone called that's always one face. Okay, it was one face. That's like the primary one. But you also sent me the OS one eye. Yeah. Right. And I'm like, do I really need to put different crap under my eyes like maybe? But then when I'm traveling, I'm like, wait a minute, I could just take the eye stuff and I could just put everything in my face. Yeah, you can. Okay. Is that a good or bad thing to do? It's not bad at all. Eye is actually is the most potent product that we have. It has a higher concentration of OS one. We actually tested in eyelid skin. So it was like specifically designed to help that skin. That's very delicate that accumulate more senescent cells. Actually, there is studies showing that the skin just under or above your eyes are 25 to 30 years older than the skin on your cheek. That's ridiculous. But you can see that right because it's way thinner. It's where you're going

46:18to accumulate more. It moves all the time. You accumulate more damage. There is more senescent cells. So from our clinical studies and even in vitro studies, it produces collagen more than double than the face product. The face is still amazing. But the eye, it's really powerful. It increases again the thickness of your skin. And from the clinical data, we saw a huge improvement in elasticity, measured with instruments, which is very accurate as well as the most accurate in clinical studies instead of just how people feel and also hydration. So seeing those improvements in a knife product with instruments plus the data that we saw in vitro of like increasing and reducing the aging markers like P16, for example, is one of the genes that we always measure to quantify the aging process. And it gets further reduced compared to face. So it's not only that sometimes people apply eye on their whole face. I don't think you need, but if you're traveling,

47:22it doesn't hurt to do that. If I can lighten the load, I really don't want to check a bag. So if I can... But do you know that your face is a, do you say friendly? Uh, face is a yes, but it's just like bigger. It's bigger. Yeah. And I also, and guys, I really use this stuff and have for a long time. So face comes with this nice white plastic thing that's big. You can pop it out. So it's a silver bag. Yeah, it's refillable. Yeah. So I just take the refillable. Yeah, I know a lot of people do that. Yeah. And it's maybe less branded that way, but it's not my goal. Yeah. I just want to carry less. But I like the size of the eye stuff. So now that you've given me permission, I'm just going to travel and put the eye stuff everywhere. And at home, I'll use the face stuff. Yeah. And I might not use the eye stuff sometimes because I've noticed a difference from that. And now this might be like a TMI moment. Like I said, it used to be really fat. And I've been on my books. I have like ripped abs. For some reason, all of my extra skin is on the back side. Right. Which isn't great. And I've done some stuff to reduce that and tighten it over time. And I've got some new tech I'm working on commercializing. And I've put like three inches of

48:26muscle on my butt. I have like the most egregious soccer butt of my life. Right. And the combination of using the one skin stuff, which rejuvenates the skin. And like so I have a lot of skin. And just having a lot more muscle there without doing any exercise at all, thank you very much. I'm like, I'm really like in the combination of just having the right the right amount of muscle and better skin. And multiple people have commented on this. So I'm not saying who commented on it. Just saying that like, yeah, actually it's my massage therapist. It's like, well, your skin is changing, which is really cool. Yeah. That product we've seen incredible results with it. And it's so interesting because it was the second product that we launched. We always had this approach of like taking care of your skin as a whole, not only your face, your skin is an organ. We needed to support your skin health as a whole. But also this is a it's a problem that a lot of people experience

49:28as they either they lose weight. And now if gelp1, we're seeing so many people using our body lotion and really helping reducing the sagging and firming their skin. But also again, through menopause, women start to experience crepness and the skin gets thinner. And the amount of like before and after is like really transformational results that we have seen. It's really exciting. And it's one product that we always want to deliver because it was important for us. But it's really meeting like a clear pain from, you know, from the consumer from our community. And it's really helping like fortify that skin. It increases the barrier of the skin. And it's also very hydrating without feeling greasy, right? Like for men like they hate like body lotion because if it feels greasy, like you can't handle. But this one it really absorbs really nice and it keeps the hydration. And you have the peptide helping build that, you know, structure or function of the skin. Very

50:30soothing, very, very like a beautiful formula that people are seeing a lot of results. And we did that clinical study in humans showing that in elderly participants when they already have a compromised barrier, we can restore their barrier with a swan body lotion. And in turn, we can reduce the inflammation systemically because your skin as it, you know, deteriorates, it becomes more, you know, accumulates more senescent cells. There is a low level of inflammation that's coming from your skin that's actually impacting your body. And by restoring that barrier, by reducing that inflammation, you are actually helping reducing terino inflammation. So it works, you know, again, beyond just the cosmetic side, which is pretty exciting. One of the aging clocks that I pay attention to is called inflammation. That looks at the effect of chronic inflammation on longevity and aging. And if you're getting 20% of your chronic inflammation from compromised

51:33skin and you can address that, it's going to affect your core biological aging as well. So I'm, I'm a fan of taking care of it, but it took some shifting for me. So I grew up in a house where I'm this is a product of, you know, the 80s and probably being in New Mexico. But it's like, you don't want to spend money on food. So, you know, let's go to Costco and buy the cheapest process, meat-like product. And you know, it was, in fact, a lot of people still think that way. Like, I don't want to spend money on food. And I also don't want to spend money on food. But I'm willing to spend money on high quality nutrition because it, it makes my brain work better, it makes my body work better. And when it comes to something like a lotion, you can buy an eight dollar lotion at Ross or something that is full of petrochemicals and angriacan disruptors and all this stuff and smell like an air freshener. But this is, you know, the, the once-kin body lotion is not a cheap lotion. It's not crazy. Yeah. But it's not cheap. But it actually works and it does something. So I just had to adjust my mental models there. I mean, it's been more than I probably would have thought about it. But it, it's because it has results. Yeah. I think we're thinking of our skin in

52:38a different lens right now. I think before, if it was just a cosmetic side, if it was just like, again, immediate or superficial hydration, you could use some of those products. But if you want to optimize the function of your skin, as you're optimizing your sleep, your nutrition, your exercise, it is an investment worth doing because you're gonna harvest these benefits like for the rest of your life. You're gonna look better, but you're, you're confident that you're putting the right thing on your largest organ, right? That largest organ should have an impact in your internal health. So it's really, really important that we are careful about it. And again, if you try to simplify and you don't need too many products, okay, you invest a few more, a little bit more, a few products, but you may be saving or not testing everything that you see on TikTok because that thing. There's a lot of stuff that just slapped together. Yeah. And what people don't understand about the skincare industry, and I'm saying this, I was an early, early investor and backer of a

53:43skincare brand maybe 14 years ago. In supplement business, there's a lot of regulation. People say it's not regulated. No, dude, it's highly regulated. And in the skincare thing, there's almost no regulation. You can smear whatever toxic crap you want on your skin and the companies are not liable. They don't have to report what it is. Like the bar is very, very low, which is why I'm extra careful about what I put on my skin. And that means like we've just the raw amount of science you've done that your clinical background in research. This is as real as it gets. And I still want to know how I track it. So I mentioned I have this expensive dermatologist equipment downstairs. But you've mentioned in a previous episode that you want something like a whoop or an aura or this new company called Avocent maybe like that you want to have a skin aging thing. Is that are we close to that? Can we take a picture with our iPhone? Like how's this going to work? Yeah, unfortunately to me, the best, the most accurate way is going to still collect a piece,

54:43little biological stamp of our skin. So we know that biopsy is not the way to go. But they are companies developing like a tape stripping method that you can collect a little bit of an upper layer of your skin. And you can understand again how your cells are functioning. Do you have to mail it in though? Yeah, you have to mail it. That's still kind of cool. So you like take a piece of tape and then end. Yeah, exactly. So there is a company in the UK that is developing that. We are doing some partnerships with them. We did like a small pilot. We already saw like really interesting reduction in biological age using their method. So we want to expand in a larger clinical study. They are still not like very, their price is still high for like a consumer. That's why it's mostly research only right now. But in the future we hope with the scale they can get there. Or even companies like Avocare. It's funny, my uncle has now passed was a co-founder of Avocent. And I'm an advisor to Avocare. And I'm like, okay, close enough words, I bring in what I'm talking. Okay. You're making the new ones can rich cream available

55:48to listeners of the show before anyone else gets access to it, which is super cool. What clinical research have you done that shows it actually works? Yeah, so I'm going to share both the invitra data that again, we spent almost two years to develop this product. And then we're going to talk about the clinicals. And we're so happy to provide this early access. Your community, you guys have been always like a huge supporter of one scan. So from our invitra data, what we got, we got an increase as we treated the scans with this rich cream, OS one rich, an increase of 28% in collagen production. And this is measuring the protein production, which is not easy to get 28% increase in collagen production in these schemes in the lab. This is within five days. Again, it's a different setting, but it just shows like the the promise of this product. We saw an increase in 77% of hyaluronic acid on the gene that produces hyaluronic acid. So it's an indirect

56:55biomarker. We saw 108% increase in the elastin gene. So the gene that will produce elastin. We saw almost 300% in the keratin tan, which is like one of the upper layer proteins that will help form the bed. What percent? Like 300? Almost 300%. Wow. So you're really changing this again. We're really supporting that the scheme to build its own barrier in a very like quantifiable way. And 37% in increase of filigrain and filigrain. It's one of the proteins that will derive the and the natural moisturizing factors. So we are giving the scheme all the structure that it needs to hold water internally as we said. And also to build that layer that to hold water on the stratum corneum, but also prevent the water loss. So really really exciting about this data. In clinicals, what we're seeing people are already seeing like 100% of our participants saw

57:58improvement in hydration. So hydration is like the staple, like everyone will get there. People are seeing they're able to hold water better over time because just providing instant hydration is easier. Make your skin to hold water. That's where it is. That's very unique of this cream. And we are also delivering these benefits on firmness and fine lines and wrinkles. So it's all 93% of the participants' improvement in firmness and 97 improvements in fine lines and wrinkles. Wow. And so for a product that's supposed to be a deep, you know, moisturizing to also be delivering these other benefits of firmness and improving fine lines and wrinkles, it's really unique and it goes back to really understanding the biology of the skin and targeting there. Are those numbers stronger than the normal one skin face? It's hard to compare because these are completely different formulations and even the

58:59clinical studies are different. So I would say that this one will offer that deeper hydration in a way and will support this building blocks in a way that's really suitable for people that need that, you know, dry skin. So if you have the dry skin, it's going to work even better. Yeah. And guys, it's oneskin.co slash Dave and you can get access to this before anyone else because you're a listener to the show. And thanks for that for our listeners. That's super cool, Karlyna. Yeah, of course. If this sounds kind of cool, I think it does. Go to oneskin.co slash Dave and you'll be able to get access to this before anyone else because the very first lot is reserved for listeners of the human upgrade. Karlyna, thank you for coming to Austin and thank you for being so smart and so completely nerdy about skin. I appreciate it. Thank you for having me again, David. Yeah, I'm so passionate about the science and just understanding the biology of

1:00:00the skin and how we can always like work to optimize it in a way that serves us better. You know, Makas look better and healthier. So thanks again for letting me have this opportunity to share more about what we do. You got it. If you liked today's episode, you know what to do. Leave a review or go out there and try one skin or drink some water, probably some salt in it. Just do something to improve yourself just a little bit today. The reason I've done more than 1400 episodes of this show over the past 12 years is probably because I get to learn from people like Karlyna, but you also get to learn and I just want you to be happy and want you to live as long as you want. I want you to feel good because I really truly believe that when you have enough energy and you do the work that you're wired in your bones and in your skin to be kind to other people. So we can make a better world just starting inside yourselves. See you next time on the Human Upgrade Podcast.

1:01:09The Human Upgrade, Formalable Approved Radio, was created and is hosted by Dave Asprey. The information contained in this podcast is provided for informational purposes only and is not intended for the purposes of diagnosing, treating, curing, or preventing any disease. Before using any products referenced on the podcast consult with your healthcare provider, carefully read all labels and heat all directions and cautions that accompany the products. Information found or received through the podcast should not be used in place of a consultation or advice from a healthcare provider. If you suspect you have a medical problem or should you have any healthcare questions, please promptly call or see your healthcare provider. This podcast including Dave Asprey and the producers, Disclaim Responsibility for any possible adverse effects from the use of information contained herein. Opinions of guests are their own and this podcast is not endorsed or accept responsibility for statements made by guests. This podcast does not make any representations or warranties about guest qualifications or credibility. This podcast may contain paid endorsements and advertisements for products or services. Individuals on this podcast may have a direct or indirect financial interest in products or services referred to herein. This podcast is owned by Bulletproof

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